BG11-PGY

 

BG-11

100 mL

Yeast Extract

250 mg

Peptone

250 mg

Glucose

250 mg

Agar

15 g

Milli-Q Water

~1 L

-Measure out approximately 300 mL Milli-Q
-Add all components in order
-Bring volume up to 1 L with Milli-Q
-Adjust pH to 7.4
-Autoclave for 20 minutes

pH: 7.4

 

BG11

NaNO3

1.5 g

K2HPO4•3H2O

40 mg

MgSO4•7H2O

75 mg

CaCl2•2H2O

36 mg

Citric Acid

6 mg

Ferric Ammonium Citrate

6 mg

EDTA (disodium magnesium)

1 mg

Na2CO3

20 mg

Trace Metal Solution

1 mL

Milli-Q Water

~1 L

-Measure out approximately 300 mL Milli-Q
-Add all components in order
-Bring volume up to 1 L with Milli-Q
-Autoclave for 20 minutes

pH: 7.4

 

Trace Metal Solution A5+Co

H3BO3

2.86 g

MnCl2•4H2O

1.81 g

ZnSO4•7H2O

222 mg

Na2MoO4•2H2O

390 mg

CuSO4•5H20

79 mg

Co(NO3)2•6H2O

49 mg

Milli-Q Water

~1 L

-Measure out 500 mL Milli-Q
-Add components in order
-Bring volume to 1 L with Milli-Q