D Medium

Stock

    NTA

    2 g

    NaNO3

    14 g

    KNO3

    2 g

    Na2HPO4

    2.2 g

    MgSO4•7H2O

    2 g

    CaSO4•2H2O

    1.2 g

    NaCl

    160 mg

    Iron Solution

    20 mg

    Micronutrient Solution

    10 mL

    Milli-Q Water

    ~1 L

    -Measure out ~500 mL Milli-Q Water
    -Add components in order
    -Bring volume to 1 L with Milli-Q
    -Adjust pH to 8.2
    -Autoclave for 20 minutes

Media

    D Medium Stock

    50 mL

    Milli-Q Water

    ~1 L

    -Measure out ~500 mL Milli-Q
    -Add D Medium Stock
    -Bring volume to 1 L with Milli-Q
    -Adjust pH to 8.2
    -Either add 1% agar, autoclave for 20 minutes and pour plates, or aliquote into erlenmeyer flasks and autoclave for 20 minutes

 

Iron Solution

FeNH4(SO4)2•??H2O

???

Na2EDTA

300 mg

Milli-Q Water

~500 mL

 

-Measure out 250 mL Milli-Q
-Add components in order
-Bring volume up to 500 mL
-Autoclave for 25 minutes

 

 

Micronutrient Solution

H2SO4

500 µL

H3BO3

500 mg

MnSO4•H2O

2.28 g

ZnSO4•7H2O

500 mg

CuSO4•5H2O

25 mg

Na2MoO4•2H2O

25 mg

CoCl2•6H2O

45 mg

Na2SeO4

4 mg

Milli-Q Water

~1 L

 

-Measure out 500 mL of Milli-Q
-Dissolve all components in order
-Bring volume to 1 L with Milli-Q