0.5 EDTA

 

Na2EDTA- dihydrate

186.1 g

Milli-Q Water

~1 L

NaOH

~20 g

-Add EDTA into 800 mL Milli-Q water (NOTE: EDTA will not dissolve)
-Using NaOH pellets, adjust pH to 8.0 (EDTA should now dissolve)
-Bring volume to 1 L with Milli-Q
-Autoclave for 30 minutes
-Store at 4oC