Microelement Stock

 

Fe-EDTA solution

80 mL

MnCl2•4H2O

180 mg

MoO3 (85% purity)
or
Na2Mo4•2H2O (99% purity)

18 mg
 
30.55 mg

ZnSO4•7H2O

22.0 mg

CuSO4•5H2O

7.9 mg

H3BO3 (Boric Acid)

286 mg

Vanadyl sufate hydrate

6.6 mg

CoC12•6H2O

4.0 mg

Milli-Q Water

~545 mL

 

-Measure out Milli-Q
-Dissolve all components in order
(Note: Do Not autoclave)

 

Fe-EDTA (A&A)

Na2EDTA•2H2O

866.7 mg

FeSO4•7H2O
or
Fe2(SO4)3•nH2O

 
2.28 mg
 

KOH

866.7 mg

Milli-Q Water

~100 mL

 

-Dissolve KOH in 31 mL Milli-Q
-Add Na2EDTA•2H2O
-In separate container, dissolve FeSO4•7H2O or Fe2(SO4)3•nH2O in 60.67 mL Milli-Q
-Mix both solutions together
-Bubble filtered air through combined solutions until the color darkens (~2.5 hr)