-Pi Stock

Microelement Stock

Fe-EDTA (A&A)

-Pi Stock

MgSO4•7H2O

20 g

CaCl2•2H2O

6 g

NaCl

20 g

Microelement Stock

500 mL

Milli-Q Water

1.5 L

-Dilute first three ingredients separately into 500 mL of Milli-Q -Autoclave three solutions, and allow to cool -Combine four solutions in order, allowing each to dissolve -Store at 4oC, shake well before use, do not autoclave

Microelement Stock

Fe-EDTA solution

80 mL

MnCl2•4H2O

180 mg

MoO3 (85% purity) or Na2Mo4•2H2O (99% purity)

18 mg 30.55 mg

ZnSO4•7H2O

22.0 mg

CuSO4•5H2O

7.9 mg

H3BO3 (Boric Acid)

286 mg

Vanadyl sufate hydrate

6.6 mg

CoC12•6H2O

4.0 mg

Milli-Q Water

~545 mL

-Measure out Milli-Q -Dissolve all components in order (Note: Do Not autoclave)

Fe-EDTA (A&A)

Na2EDTA•2H2O

866.7 mg

FeSO4•7H2O or Fe2(SO4)3•nH2O

2.28 mg

KOH

866.7 mg

Milli-Q Water

~100 mL

-Dissolve KOH in 31 mL Milli-Q -Add Na2EDTA•2H2O -In separate container, dissolve FeSO4•7H2O or Fe2(SO4)3•nH2O in 60.67 mL Milli-Q -Mix both solutions together -Bubble filtered air through combined solutions until the color darkens (~2.5 hr)

MgSO₄•7H₂O

CaCl₂•2H₂O

-Dilute first three ingredients separately into 500 mL of Milli-Q
-Autoclave three solutions, and allow to cool
-Combine four solutions in order, allowing each to dissolve
-Store at 4^oC, shake well before use, do not autoclave

MnCl₂•4H₂O

MoO₃ (85% purity)
or
Na₂Mo₄•2H₂O (99% purity)

18 mg

30.55 mg

ZnSO₄•7H₂O

CuSO₄•5H₂O

H₃BO₃ (Boric Acid)

CoC₁₂•6H₂O

-Measure out Milli-Q
-Dissolve all components in order
(Note: Do Not autoclave)

Na₂EDTA•2H₂O

FeSO₄•7H₂O
or
Fe₂(SO₄)₃•nH₂O

-Dissolve KOH in 31 mL Milli-Q
-Add Na₂EDTA•2H₂O
-In separate container, dissolve FeSO₄•7H₂O or Fe₂(SO₄)₃•nH₂O in 60.67 mL Milli-Q
-Mix both solutions together
-Bubble filtered air through combined solutions until the color darkens (~2.5 hr)