25% Ringer's Solution

 

NaCl

2.25 g

KCl

105 mg

CaCl2

45 mg

NaHCO3

50 mg

Citric Acid

34 mg

Milli-Q Water

~1 L

-Measure out approximately 300 mL Milli-Q
-Add all components in order
-Bring volume up to 1 L with Milli-Q
-Autoclave for 45 minutes