TE Buffer

 

1M Tris

5 mL

0.5M EDTA

1 mL

Milli-Q Water

~500 mL

-Measure out 490 mL Milli-Q
-Add components in order
-Adjust pH to 7.4 using HCl
-Bring volume to 500 mL
-Autoclave for 30 minutes
-Store at 4oC

 

1M Tris

Tris Base

121.1 g

Milli-Q Water

~1 L

HCl

~42 mL

-Dissolve Tris base into 800 mL Milli-Q water
-Using HCl, adjust pH to 8.0
-Bring volume to 1 L using Milli-Q
-Autoclave for 30 minutes (NOTE: re-make if solution turns yellow)
-Store at 4oC

 

0.5M EDTA

Na2EDTA- dihydrate

186.1 g

Milli-Q Water

~1 L

NaOH

~20 g

-Add EDTA into 800 mL Milli-Q water (NOTE: EDTA will not dissolve)
-Using NaOH pellets, adjust pH to 8.0 (EDTA should now dissolve)
-Bring volume to 1 L with Milli-Q
-Autoclave for 30 minutes
-Store at 4oC