TESC

NaCl	87.66 g
1M Tris	100 mL
0.5M EDTA	200 mL
Hexadecyltrimethylammonium Bromide (CTAB)	10 g
Milli-Q Water	~1 L

-Measure out approximately 500 mL Milli-Q -Add all components in order -Bring volume up to 1 L with Milli-Q -Adjust pH to ~8.0 -Autoclave for 30 minutes -Store at room temperature

1M Tris

Tris Base	121.1 g
Milli-Q Water	~1 L
HCl	~42 mL

-Dissolve Tris base into 800 mL Milli-Q water -Using HCl, adjust pH to 8.0 -Bring volume to 1 L using Milli-Q -Autoclave for 30 minutes (NOTE: re-make if solution turns yellow) -Store at 4^oC

Na₂EDTA- dihydrate	186.1 g
Milli-Q Water	~1 L
NaOH	~20 g

-Add EDTA into 800 mL Milli-Q water (NOTE: EDTA will not dissolve) -Using NaOH pellets, adjust pH to 8.0 (EDTA should now dissolve) -Bring volume to 1 L with Milli-Q -Autoclave for 30 minutes -Store at 4^oC