| The Harrison lab occupies approximately 1200
square feet in the Interdisciplinary Science and Technology Building
1 (opened Feb. 2006) on the main campus of Arizona State University,
Tempe.
The main lab includes on-tap research grade water
and a separate room for insect rearing and an additional separate
fume hood alcove. The lab includes a built-in purge gas generator
for respirometry, and extensive tank holders for gas mixing. In
addition to the main lab, common facilities include a walk-in
environmental room, a washroom with autoclave and dishwashers,
and an equipment room with gel readers, deep-freeze and centrifuges.
Equipment classes:
Insect rearing in hypoxia/hyperoxia:
Four refrigerator-style low temperature (4-50∞C)
incubators
Two Sable Systems ROXY-8 (regulates and records oxygen kPa for
up to 8 chambers)
Eight 0.5 m3 gas-tight plexiglass rearing chambers
Eight 1 m3 gas-tight Lexan rearing chambers
Balances, stir-plates, and glassware for preparing insect media
Micro- and macro-morphological measurements:
Sartorius MC5 Microbalance
Mettler AE240
Cahn C-33 microbalance
Digital micrometers
3 Cambridge Instruments Stereozoom microscopes
Hitachi HVC20M 3-CCD camera for digital imaging
Surgipath Rotary Microtome
American Optical compound microscope
Canon Digital Rebel camera
2 Canon Digital Camcorders
Panasonic AG-456 S-VHS Movie Camera, Desktop Editor
We also have access to the SOLS Keck Imaging Laboratory, with
multiple compound and confocal microscopes with CCD cameras
Gas exchange measurements, gas mixing:
Balston 75-52 FT-IR purge gas generator
LiCor 6262 CO2/H2O analyzer
2 LiCor 6252 CO2 analyzers
Anarad AR-411 CO2 analyzer
Sable Systems Oxzilla oxygen analyzer
Ametek S3A oxygen analyzer
Sable Systems FOXBOX (oxygen analyzer, mass flow controller)
Micro-electrode Inc. Picoammeter and oxygen electrode
Thunder Scientific H2O sensor
Brooks 5850i mass flow controllers and four 5878 mass control
units
A wide variety of mass flow meters, controllers, flowmeters,
gas regulators, and flow calibration units
Data Acquisition/Analysis
5 Sable Systems data acquisition systems of varying age
Multiple preamplifiers and signal conditioners
Multiple personal computers
SAS, Statistica, Systat, SigmaPlot, MatLab
Frequency, Flow, Temperature and Pressure
measurement
Custom-built Optical Tachometer (measurement of
insect wingbeat frequencies)
Sable Systems Optical Tachometer
Sable Systems PT-1000 pressure transducer
Physitemp BAT 10 and BAT 12 thermocouple thermometers
Omega PX164005BD5V pressure transducer with custom electronics
(measurements of pressure fluctuations in insect tracheal systems)
Custom-built optical ventometer for measuring insect abdominal
volumes changes during ventilation
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