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Ecological & Evolutionary Physiology

Harrison Lab Facilities

The Harrison lab occupies approximately 1200 square feet in the Interdisciplinary Science and Technology Building 1 (opened Feb. 2006) on the main campus of Arizona State University, Tempe.

The main lab includes on-tap research grade water and a separate room for insect rearing and an additional separate fume hood alcove. The lab includes a built-in purge gas generator for respirometry, and extensive tank holders for gas mixing. In addition to the main lab, common facilities include a walk-in environmental room, a washroom with autoclave and dishwashers, and an equipment room with gel readers, deep-freeze and centrifuges.


Equipment classes:

Insect rearing in hypoxia/hyperoxia:

Four refrigerator-style low temperature (4-50∞C) incubators
Two Sable Systems ROXY-8 (regulates and records oxygen kPa for up to 8 chambers)
Eight 0.5 m3 gas-tight plexiglass rearing chambers
Eight 1 m3 gas-tight Lexan rearing chambers
Balances, stir-plates, and glassware for preparing insect media

Micro- and macro-morphological measurements:

Sartorius MC5 Microbalance
Mettler AE240
Cahn C-33 microbalance
Digital micrometers
3 Cambridge Instruments Stereozoom microscopes
Hitachi HVC20M 3-CCD camera for digital imaging
Surgipath Rotary Microtome
American Optical compound microscope
Canon Digital Rebel camera
2 Canon Digital Camcorders
Panasonic AG-456 S-VHS Movie Camera, Desktop Editor
We also have access to the SOLS Keck Imaging Laboratory, with multiple compound and confocal microscopes with CCD cameras

Gas exchange measurements, gas mixing:

Balston 75-52 FT-IR purge gas generator
LiCor 6262 CO2/H2O analyzer
2 LiCor 6252 CO2 analyzers
Anarad AR-411 CO2 analyzer
Sable Systems Oxzilla oxygen analyzer
Ametek S3A oxygen analyzer
Sable Systems FOXBOX (oxygen analyzer, mass flow controller)
Micro-electrode Inc. Picoammeter and oxygen electrode
Thunder Scientific H2O sensor
Brooks 5850i mass flow controllers and four 5878 mass control units
A wide variety of mass flow meters, controllers, flowmeters, gas regulators, and flow calibration units

Data Acquisition/Analysis

5 Sable Systems data acquisition systems of varying age
Multiple preamplifiers and signal conditioners
Multiple personal computers
SAS, Statistica, Systat, SigmaPlot, MatLab

Frequency, Flow, Temperature and Pressure measurement

Custom-built Optical Tachometer (measurement of insect wingbeat frequencies)
Sable Systems Optical Tachometer
Sable Systems PT-1000 pressure transducer
Physitemp BAT 10 and BAT 12 thermocouple thermometers
Omega PX164005BD5V pressure transducer with custom electronics (measurements of pressure fluctuations in insect tracheal systems)
Custom-built optical ventometer for measuring insect abdominal volumes changes during ventilation

 

Flight metabolism
Why Study Insects?
Insect nutritional ecology
Facilities and methods
Cyborg Insects

 

© 2007 Jon F. Harrison, School of Life Sciences. All rights reserved.
School of Life Sciences Arizona State University