This is the web page of Professor J.C.H. Spence in the Physics Department at Arizona State University, U.S.A. Research is conducted in our ASU lab, at LBNL, Berkeley, and at LCLS at SLAC on atomic structure and processes in organic and inorganic matter. It is funded by NSF, DOE, ARO and LBL. Updated 4/13.
His faculty web page can be found at http://physics.asu.edu/people/faculty
Our research group web page is http://spencelab.la.asu.edu/
Our addresses are as follows:
Tel. USA 602-965 6486. Lab: 602-965 2535, Fax USA 602-965 7954
Tel: 510-486-7775, 510-486-4067. Fax 510-486-7696.
John Spence completed a PhD in Physics at Melbourne University in 1972, followed by a postdoc at Oxford UK. He is a Fellow of the American Association for the Advancement of Science, the American Physical Society, the Microscopy Society of American, of the Institute of Physics (UK), and of Churchill College Cambridge, UK. He received the MSA Distinguished Scientist award for 2006 and the Buerger Award of the American Crystallographic Society in 2012. He was Co-Editor of Acta Cryst (A) for North America (Diffraction Physics, 1990-2000), and served recently on the Scientific Advisory Committee for the Advanced Light Source at LBNL. He is a member of the DOE BESAC Committee, and of the Molecular Foundry Advisory Board at Lawrence Berkeley Laboratory, and was a Member of US National Committee for Crystallography , Recipient of the Burton Medal of the Microscopy Society of America and of a Humbolt Senior Scientist award. He is an editorial board member for the Oxford University Press series on Mesoscopic Physics, of Reports on Progress in Physics, of Ultramicroscopy and J. Micros. He was chair of the International Union of Crystallography Commission on Electron Diffraction, a member of the IUCr commission on Charge, Spin and Momentum densities and was chair of the Gordon Conference on Charge, Spin and Momentum densities for 2004, and recipient of the Frontiers In Electron Microscopy Lectureship award for 2003 and FAST Lecturer at Cornell in 2005. A Festschrift volume of Ultramic appeared in July 2011. The Xray laser structural biology work was ranked among the top ten scientific breakthroughs of 2012 by Science magazine.
Some recent papers
-1. "X-ray lasers for structrual and dynamic biology". Rep Prog Phys (2012). J. Spence, U. Weierstall and H. Chapman. 75, 102601
0. "Femtosecond protein nanodiffraction using an X-ray laser" H. Chapman, P. Fromme........J.C.H.Spence. Nature 470, 73-77. (2011)
1. On Jia et al seeing Oxygen in Superconductors. Science 03. http://www.public.asu.edu/~jspence/JCHS_Science_03.pdf
2. On Kiesel et al - the electron antibunching effect observed. Nature 02 http://www.public.asu.edu/~jspence/N&V_Antibunch_JCHS_02.pdf
3. On X-ray holograms of atoms...Nature 01 . http://www.public.asu.edu/~jspence/N&V_SpenceNature'01.pdf
4. Electron-excited fluorescence makes hologram of atomic structure. J. Spence et al. PRL 01 http://www.public.asu.edu/~jspence/PRLSpenceKoch01.pdf
5. "Imaging" of the chemical bonding in copper oxide. J. Zuo et al. Nature 98. http://www.public.asu.edu/~jspence/NatureBonding.pdf
6. "Direct observation of dislocation kinks and their motion". H. Kolar et al. PRL 96. http://www.public.asu.edu/~jspence/KolarPRLKinks.pdf
7. "Single Molecule Diffraction". J.C.H.Spence and B.Doak. Physical Review Letters. 92, 198102 (2004)
8. "Motion of laser-aligned hydrated proteins in a beam" D. Starodub et al. J. Chem Phys 123 244304 (2005)
9. "Femtosecond Protein Nanocrystallography - data analysis methods". R. Kirian et al Optics Express 18, 5713 (2010).
Powerpoints from the recent workshop on "Biology with X-ray lasers" at LBNL can be found at https://sites.google.com/a/lbl.gov/biology-with-fels/
Conference at Royal Society , London , Oct 14, 2013 on use of X-ray Lasers in Biology - http://royalsociety.org/events/2013/x-ray-lasers/
Students in this group work toward Physics, Materials Science or Biophysics MSc and PhD. degrees under the supervision of Prof. Spence, in the following areas:
New electron optical instruments are also under development such as our Scanning Atom Probe, the Point Reflection Electron Microscope, the laser aligned, hydrated protein beam, hydrated sample injectors for the LCLS X-ray laser (in collaboration with B. Doak, U. Weierstall, P. Fromme, H. Chapman and others), diffraction physics related to XFELs, and Microdiffraction RHEED.
Summaries of some of our work can be found in the books "High Resolution Electron Micrsocopy" (J.C.H. Spence, Oxford Univ. Press, 2013, 4th edition) and "Electron Microdiffraction" (J. Spence and J. Zuo, Plenum, 1992). The book "Science of Microscopy" (2 Vols, Eds P. Hawkes and J.C.H.Spence) , Springer, 2007, reviews all forms of modern microscopy.
Students or postdoctorals interested in visiting should contact us through Internet.
Some recent papers (updated Jan 2012.)
Condensed Matter, Ab-initio Quantum Molec Dynamics for cracks,
We predict the mechanical properties (fracture toughness, ductility) of perfect crystals using atomistic quantum mechanical calculations.
25. "Electronic structure of the unreconstructed 30 partial dislocation in silicon", J.E. Northrup, M.L. Cohen, J.R. Chelikowsky, J. Spence and A. Olsen, Phys. Rev. B24, 4623-4628 (1981).
201 Atomistics and mechanical properties of silicon. J.C.Spence. Acta Mat et Met. (1999). 47, p. 4153.
81. "A Kink Mechanism for Formation of the Si(111)-(2x1) Reconstructed Surface", J. Spence. Phys. Rev. B38, p. 12672-12674 (1988)
105. "The influence of internal surfaces on the (2x1) shuffle and glide cleavage reconstructions" Y.M.Huang, J.C.H.Spence, O. Sankey, and G. Adams. Surface Science (1991) 256, p.344. URI
146. "Dislocation kink motion in silicon". Y. M. Huang, J.C.H.Spence and O. Sankey. Phys. Rev. Letts. 74, p. 3392 (1995) .
120. "Lattice trapping and surface reconstruction for silicon cleavage on (111). Ab- initio quantum molecular dynamics calculations". J. Spence, Y. Huang and O.Sankey. Acta Met. 41, p. 2815 (1993).
46. "High energy transmission electron diffraction and imaging studies of the silicon surface", J.C.H.Spence, Ultramic., 11, 117 (1983).
139 "The effect of impurities on the ideal tensile strength of silicon" Y. Huang, J. Spence and O. F Sankey, Phil Mag. 1994. 70, p.53.
120. "Lattice trapping and surface reconstruction for silicon cleavage on (111). Ab- initio quantum molecular dynamics calculations". J. Spence, Y. Huang and O.Sankey. Acta Met. 41, p. 2815 (1993)
_Protein structure and dynamics by femtosecond X-ray diffraction from a liquid jet
of hydrated molecules (at the LCLS XFEL).
We form molecular movies (eg of photosynthesis with P. Fromme) by optical laser excitation followed by snapshot hard X-ray laser diffraction.
439 ""X-ray lasers for structural and dynamic
biology". J.C.H.Spence, U. Weierstall and H.N.Chapman. Rep. Prog. Phys. 75,
102601 (2012) .
"Single Molecule Diffraction". J.C.H.Spence and B.Doak. Physical Review Letters. 92, 198102
"Diffraction and imaging from a beam of laser-aligned proteins". J.C.H.Spence et al. Acta Cryst A61, p.237. (2005) see also J. Chem Phys. 123, 244304 (2005).
372 "Gas dynamic virtual nozzle for generation of microscopic droplet beams". D. De Ponte et al. J. Phys. D. Appl Phys. 41, 195505 (2008)
401 "Femtosecond protein nanocrystallography - data analysis methods". R. Kirian et al Optics Express 18, p.5713 (2010).
408 "Femtosecond protein nanocrystallography". H. Chapman, P. Fromme...... J.C.H.Spence. Nature 470, p73-77 (2011).
409 "Single mimivirus particles imaged on-the-fly with an X-ray laser" M. Seibert....J. Hajdu. Nature 470, p. 78 (2011)
412 "Phasing of coherent femtosecond X-ray diffraction from size-varying nanocrystals". J.C.H.Spence et al Optics Express 19, 2866-2873 (2010)
425 "Signal , noise and resolution in correlated flucuations from snapshot SAXS" R.Kirian et al. Phys Rev E (2011) submitted.
Ordering in Glasses.
" The most important unsolved problem in Condensed Matter Physics" (P. Anderson).
261 "Long range structural fluctuations in a CaO-Al2O3-2SiO2 glass by spatially resolved near-edge spectroscopy. N. Jiang, J. Qiu and J.C.H.Spence. Phys Rev B66, p.054203 (2002).
Organic materials, Cryo-electronmicroscopy.
288 Three-dimensional diffractive imaging for crystalline monolayers with one-dimensional compact support. J.Spence, U. Weierstall, K. Downing and R. M. Glaeser. J. Struct. Biol. 144, 209. 2003.
262 Kinematic and dynamical CBED for solving thin organic films at low temperature; experimental tests with anthracene. J.S. Wu and J.C.H. Spence. Acta Cryst A58, p.580. (2002)
399 "Design of an electron microscope phase plate using a focussed continuous-wave laser". H. Muller et al. New J Phys. 12, 073011 (2010).
Bonding in solids. Electron Microdiffraction.
We develop new methods for imaging the chemical bonds which glue atoms together in solids, and test many-electron theoretical approximations for bonding.
177."Charge density of MgO: Implications of precise new measurements for theory". J.M.Zuo, M. O'Keeffe, P. Rez, J. Spence. Phys Rev Letts. 78, p. 4777-4780. (1998)
174 "Direct inversion of dynamical electron diffraction patterns to structure factors". J. Spence. Acta Cryst. A. 1998. A54 p. 7 - 18.
153 "Effect of Mn doping on chanrge density in g-TiAl by quantitative CBED" R. Holmestad, J. Zuo, J. Spence R Hoier and Z. Horita. Philos. Mag. (1995) 72, p. 579
28 "A simple method for determining structure factor phases and crystal polarity using electron diffraction", J. Tafto/ and J. Spence, J. Appl. Cryst., 15, 60-64 (1982),
82. "Bonding in GaAs", J.M. Zuo, J. Spence and M. O'Keefe. Phys. Rev. Letts Vol. 61, p353 (1988)
90. "Accurate structure-factor phase determination by electron diffraction in acentric crystals", J.M. Zuo, J. Spence and R. Hoier. Phys. Rev. Letts. (1989) 62 p. 547.
103 "Charge ordering in magnetite at low temperatures". J.M.Zuo, J.C.H. Spence and W.Petusky. Phys. Rev. B.42, p.8451 (1990).
218 "On the measurement of dislocation core periods by nanodiffraction". J.C.H.Spence and C. Koch. Phil Mag. B.81, 11,pp.1701-1711(2001). (Bourret Festschrift).
104. Bonding in copper oxide - direct observation of chemical bonds."Direct Observation of d holes and Cu-Cu bonding in Cu2O". J. Zuo, Y.Kim, M. O'Keeffe, J. Spence. Nature Vol 401, p.49, (1999). 226 "On closed shell interactions, polar covalences, d shell holes, and direct images of orbitals: the case of cuprite". J.Zuo, M.O’Keeffe, M.Kim, J.Spence. Angew Chem Int Ed 2000, 39, p. 3791. See http://www.public.asu.edu/~jmzuo/pics/pics.htm. For images, click below. Nature99CopperOxideBonds
Solving crystal structures by
387 "The kinematic convergent beam method for solving nanocrystal structures". J. McKeown and J.C.H.Spence. J. Appl Phys. 106, 074309 (2009)
Charged particles buzzing through crystals generate tunable coherent X-ray with the buzz frequency.
68. "Pendellosung Radiation and Coherent Bremsstrahlung", J.C.H. Spence and G. Reese, Acta Cryst. (P. Ewald Festschrift) 1986 Acta Cryst. A42, p. 577-585, NSF
Electron energy -loss spectroscopy.
Provides similar information to soft-Xray absorption spectra, but with nanometer spatial resolution.
2. "Observation of double plasmon excitation in aluminium", J.C.H. Spence and A.E.C. Spargo, Phys. Rev. Letters 26, 985 (1971).
70. "The electron energy loss near edge structure of Be2C", M.M. Disko, J.C.H. Spence, O.F. Sankey and D. Saldin. Phys. Rev. B. (1986) B33, p. 5642-51, ARO 83.
33. "STEM Microanalysis by Transmission Electron Energy Loss Spectroscopy in Crystals", J.C.H. Spence and J. Lynch, Ultramicroscopy, 9, 267 (1982), ARO.
60. "The Structural Sensitivity of Electron Loss Near Edge Structure", J.C.H. Spence, Ultramicroscopy (1985), 18, p. 165-172, ARO 83.
Channeling effects on secondary processes, internal source holography
Electron standing waves in crystals can be used to locate foreign dopant atoms.
47. "ALCHEMI - a new technique for locating atoms in small crystals", J.C.H Spence and J. Tafto, J. Micros, 130, 147 (1983), NSF 80.
79. "Adatom site determination using channelling effects in RHEED on X-ray and Auger electron production", J.C.H. Spence and Y. Kim (1988). in "Reflection High Energy Electron Diffraction and Reflection Electron Imaging of Surfaces" Eds. P.K. Larsen and P.J. Dobson, p. 117-128 Plenum1988 NSF 85.
"Atomic String Holography". J. Spence and C. Koch. Phys. Rev. Letts. 86, p. 5510 (2001).
In-situ observation of STM operation in a TEM
Manipulate atoms by STM and watch simultaneously by TEM
124 Investigation of STM image artifacts by in-situ reflection electron microscopy. W. Lo and J. Spence. Ultramic. (1992). 48, p.433.
Atomic-scale imaging of defects, ordering. High Tc, Dislocation kink
The atomic structure of defects controls many materials properties.
17. "Distinguishing dissociated Shuffle and Glide set dislocations by high resolution electron microscopy", A. Olsen and J.C.H. Spence, Phil. Mag. A43, 945-965 (1980)
154 "Observation of moving dislocation kinks and pinning". H. Kolar, J. Spence and H. Alexander. Phys. Rev. Letts. 77, p. 4031-4034 (1996) . KinkImages
74. "Microstructure, Oxygen Ordering and planar defects in in high Tc superconductor Ba2YCu3O6.9", A. Ourmazd, J.A. Rentschler, J.C. Spence, M. O'Keeffe, R.J. Graham, D.W. Johnson and W.W. Rhodes. Nature (1987) 327, p. 308, NSF 85.
9. "Lattice imaging in STEM", J.C.H. Spence ,J.M. Cowley, Optik, 50, 129 (1978).
170 "The enhancement of electron microscope resolution by use of atomic focusers". J. M. Cowley, J. Spence and V.V. Smirnov. Ultramic. 68, p. 135 (1997).
200. Electron nanodiffraction for dislocation kinks and core reconstruction". C. Koch, J.Spence et al.. J. Phys (Cond. Matter) 12, p. 1-9 (2000).
281 "Oxygen in crystals - seeing is believing". Science. 299, p. 839 (2003) "Perspective".
Field emission nanotips, electron antibunching, low energy electron
holography in biology, photofield emission.
131 "Brightness measurement of nanometer sized field emission electron sources". W. Qian, M. Scheinfein and J. Spence. J. Appl. Phys. 73, 7041 (1993).
138 "Electron source information from Fresnel fringes in field emission point projection electron microscopy" J. Spence= et al.. J. Vac Sci. A12. p.542 (1994)
122 Aberrations of emission cathodes: Nanometer diameter field emission electron sources. M. Scheinfein, W, Qian and J, Spence. J. Appl. Phys. 73 (1993) p.2057
187 "Imaging of Tobacco Mosaic Virus at 40 volts by electron holography". U. Weierstall and J. Spence. Micron 30, p.335 (1999). ARO.
232 "Electron Holography of field-emitting carbon nanotubes". Cumings, Zettl, McCartney, Spence. Phys Rev Letts. 88, p.056804 (2002)
266 "Spaced out electrons". Electron antibunching. News and Views. Nature 418, p. 377 2002. J.C.H. Spence.
396 "A coherent photofield electron source for fast diffractive and point-projection imaging". J.C.H. Spence, T. Vecchione and U. Weierstall. Phil Mag. Accepted (2010)
New electron detectors.
86. "A large dynamic range parallel detection CCD system for electron diffraction and imaging", J.C.H. Spence and J.M. Zuo, J. Sci. Instr. 59(9), p. 2102-2105 (1988)
Cathodolluminescence in STEM
Atomic-level spatial resolution with the energy resolution of optical spectroscopy.
54. "Cathodoluminescence and polarization studies of dislocations in diamond", N. Yamamoto, J.C.H. Spence and D. Fathy, Phil. Mag B49, p. 609-629 (1984).
Electron holography. Ferroelectrics
Electron holography allows one to map out electric and magnetic fields in solids.
160 "Theory and principles of electron holography" J. Cowley, J. Spence. in : Intro to electron holography. Eds Allard et al. Plenum (NY) 1996
129 "Electron holographic study of ferroelectric domain walls" J.C.H.Spence, J.M. Cowley and J.M. Zuo. Appl. Phys. Letts. 62, p. 2446 (1993)
232 "Electron holography of field-emitting carbon nanotubes". Cummings, Zettl, McCartney, Spence. Phys. Rev. Letts. 88, p.056804 (2002).
The Scanning Tunnelling Atom Probe.
Our machine combines an STM with a time-of-flight atom probe.
189 "Atomic species identification in STM using an imaging atom-probe technique". U. Weierstall and J. Spence. Surf Sci. 398 p. 267-279.
Inversion problem of multiple electron or X-ray scattering.
Direct, non-iterative methods for recovering potentials from multiple-scattering data.
174. "Direct inversion of dynamical electron diffraction patterns to structure factors". J. Spence. Acta Cryst. A. 1998. A54 p. 7 - 18.
3. "Determination of single-scattering distribution from multiple scattering data in ELS", D Johnson, and J. Spence, J.Phys. D.7, 771 (1974).
256 "A useful disentanglement of the exponential of the sum of two non-commuting matrices, one of which is diagonal". C. T. Koch and J.C.H.Spence. J. Phys A36, 803 . (Math Phys. UK) 2003.
382 "Two-wavelength inversion of multiply-scattered soft X-ray intensities to charge density". J.C.H.Spence. Acta Cryst A65. p.28-38 (2009)
X-ray holography, diffraction-imaging, lensless imaging and powder
diffraction by iterative phasing.
Images can now be reconstructed from far-field scattering from non-periodic objects. Applications to femtosecond X-ray diffraction from proteins or single particles.
241 "Workshop on "New approaches to the Phase Problem for non-periodic objects" John C.H.Spence, M. Howells, L.D.Marks and J. Miao. Ultramic. 90, p.1-6 (2001).
237. "Holography of atoms". J.C.H.Spence. Nature. 410, p.1037. (2001).
249 "Phase recovery and lensless imaging by iterative methods in optical and electron diffraction". J..Spence, U. Weierstall ,M. Howells. Phil. Trans. 360, p.875 (2002).
289 " X-ray image reconstruction from a diffraction pattern alone. S. Marchesini, J.C.H. Spence et al... Phys Rev B68, 140101(R)
343 "Ab initio phasing of X-ray powder diffraction patterns by charge flipping". J.S.Wu, J.C.H.Spence, M. O'Keeffe, K. Leinenweber. Nature Materials. 5, 647 2006.
367 "Dose, exposure time and resolution in serial crystallography". D. Starodub, J. Spence et al. J. Synch Res. 15, 62-73 (2008)
388 "Triple-beam tomographic femtosecond X-ray diffractive imaging". K. Schmidt, J. Spence, U. Weierstall, D. Starodub, H. Chapman, M. Howells, B. Doak.J. Phys Rev Letts. 101, 115507 (2008)
386 "Powder diffraction from a continuous microjet of nanoscale protein crystals". D.A. Shapiro, D. De Ponte, R.B. Doak, P. Fromme, G. Hembree, M. Hunter, S. Marchesini, K. Schmidt, U. Weierstall, J.C.H.Spence. J. Synch. Rad. 15, 593-599 (2008). (2008).
377 "Ultrafast, ultrabright, X-ray holography using a uniformly redundant array". S.Marchesini J.C. H. Spence et al. (2008) Nature Photonics 2, 560 - 563 (2008).