Week 1

1. Restriction digests of plasmids isolated by TA’s.

2. Preparation of competent cells.

3. Pour plates for transformation.

4. Recover DNA from restriction digests.

Week 2

1. Ligate DNA from restriction digests.

2. Gel analysis of restriction digests.

3. Transform bacteria with ligation mixtures.

Week 3

1. Isolate plasmid from transformed bacteria.

2. Restriction digest of isolated plasmids.

3. Gel analysis of restriction digests.