RESOURCES: Cell Culture

• Mammalian Cell Culture Resource: (Goldwater Building)

The core cell culture facility has been set up to contain all of the necessary equipment for mammalian cell culture. It contains basic equipment for microbial growth in the event that vectors are required for transfection. The facility occupies GWC 627, 627a, 627b, 627c and part of 631. The main room is reserved for standard cell culture. It contains four, 4 foot biosafety cabinets, 2 dual chamber CO2 incubators, 2 microgravity bioreactors, 2 Nikon light microscopes with digital imaging including Cohu cameras and one Nikon light microscope without imaging. The analytical and microbial areas contain instruments such as a dual beam UV/Vis spectrophotometer, micro‑ultra centrifuge , a speed‑vac unit, sonicators,  thermocycler, microbial incubators, and one 20‑liter lyophilizer. The third area is the transfection room,.  http://www.eas.asu.edu/~bme/ (Click on facilities).

• Cell Biotechnology Laboratory: (LSA Building)

This resource is available principally to students and faculty at Arizona State University.  Industrial users can approach the “Mammal Cell Culture Resource” described above.  This laboratory was made possible by grants from the Howard Hughes Foundation (in support of undergraduate research) and the Whitaker Foundation (Program Development Award).  The laboratory’s principal function is to create an environment to jointly train advanced undergraduate students and beginning graduate students from both the Life Science and Bioengineering Departments in a formal course setting where the students can learn the paradigms, vocabulary, and limitations of each discipline, as well as promoting team work.  Students gain experience in mammalian cell culture, the growth of mouse embryonic stem cells, the use of microgravity bioreactors and microcapillary bioreactors, and a variety of other techniques in cell biology.  This laboratory is in use year round by students.  http://lsvl.la.asu.edu/bio494/dcapco/  and http://www.eas.asu.edu/~bme/ (click on facilities)

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