Current funding at Arizona State University
- Interaction of Photosystem I with its natural electron acceptor ferredoxin: a model system for protein recognition and inter-protein electron transfer
Principle investigator: P. Fromme
Source of funding: United States Department of Agriculture 2003-35318-13573
Funding Period 08/2003 to 08/2006
This grant provides support for the study of the interaction of Photosystem I with ferredoxin. The research involves the improvement of the PS I-ferredoxin crystals, X-ray structure analysis as well as EPR spectroscopic investigations on the complex
- The mechanism of water splitting in PS II investigated by X-ray crystallography and spectroscopy
Principle investigator: P. Fromme; co-Pi's R. LeBrutto (ASU, SOLS) and D. Britt (UCLA)
Source of support: NIH R01 GM7 1619-01
This grant provides support for the investigation of the structure and function of Photosystem II, a large membrane protein complex, which produces all the oxygen in the atmosphere. The protein complex will be crystallized and the structure determination will be performed to atomic resolution by X-ray structure analysis. The structural studies are complemented by functional investigations using Electron Paramagnetic Resonance (EPR) techniques. The aim of the project is to unravel the mechanism of water splitting.
- Structure and function of supercomplexes of Photosystem I with its peripheral antenna systems in green algae and cyanobacteria
Principle investigator: P. Fromme; co-Pi: A. Webber (ASU, SOLS) and A. Melkozernov (ASU Chemistry and Biochemistry)
Source of support: NSF MCB 04127142
This grant provides support for the investigation of the structure and function of supercomplexes of Photosystem I with its antenna complexes. Photosystem I is a large membrane protein complex consisting of 12 proteins to which more than 100 cofactors are bound. Peripheral antenna complexes are functionally and structurally coupled to Photosystem I to further increase the antenna size for optimal light capturing. The aim of the project is the stabilization of the super-complexes by side directed mutagenesis, crystallization of the supercomplexes, determination of their structure by X-Ray structure analysis and functional investigations by ultra-fast spectroscopy.
- NSF98-119 Serial crystallography
Principle investigator: J.C.H. Spence (ASU Physics) coPI's : B. Doak (ASU Physics), : P. Fromme (ASU Chem. and Biochem.), U.Weierstall (ASU Physics), G. Hembree (ASU Physics) , K. Schmidt (ASU Physics)
Source of support: SGER-NSF
This grant provides initial funding for the first steps in the development of a new technique for the structure determination of proteins by electron diffraction of oriented single molecules. The first part of the project includes the development of a stream of nano-heliumparticles, each of them including one protein molecule covered by a shell of amorphous ice. The next step will be the orientation of the particles in a laser beam and the detection of the electron diffraction of the stream of oriented molecules. The diffraction of up to 10 10 oriented molecules will be thereby detected to provide a diffraction pattern. The final aim of the project is to determine of protein- structures by single molecule electron diffraction.
Previous funding at the Technical University Berlin
- Advanced diagnostics and operation concepts for crystal growth of bio-molecules under microgravity
Principle investigators: P. Fromme, N. Chayen and K. Dierks and Partner Systemtechnik, Hamburg funded by the European Space agency SA-Grant Period: 11/01/2000 to 10/31/2003
This grant provided support for the development of a new type of crystallization-reactor for the crystallization of bio-molecules under microgravity at the International Space Station. The aim of the project is to develop a crystallization reactor that allows full online control of the crystallization parameter in orbit and includes seeding options.
- New Developments for Crystallization of Photosynthetic Membrane Proteins under microgravity
Principle investigator: P. Fromme funded by the Germany Space Agency ( Deutsche Gesellschaft für Luft und Raumfahrt) and the Bundesministerium fuer Forschung BMBF (Minister of Research) Period: 10/01/2000 to 12/31/2003
This grant provides support for the research on the nucleation and crystallization processes during crystallization of membrane proteins under microgravity. The main aim was to grow large well ordered single crystals of Photosystem I during the STS-107 mission of the space shuttle Columbia.
- Crystallization of the water-oxidizing Photosystem II for structural and functional analysis of the system
Principle investigators: Petra Fromme and H.T Witt,funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the Special Research Unit 498Protein-Cofactor-Interactions in Biological Systems, project C3 , Period: 01/01/1999 to 12/31/2002
This grant provided support for structural and functional investigations on the Photosystem II. The project includes crystallization of Photosystem II, X-ray structure analysis and spectroscopic investigations on the single crystals.
- Structure and Function of Photosystem I
Principle investigators: Petra Fromme, Eberhard Schlodder, H.T. Witt funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the Special Research Unit 498Protein-Cofactor-Interactions in Biological Systems, project A1 , Period: 01/01/1999 to 12/31/2002
This grant provided support for the structure determination of Photosystem I by the method of X-ray crystallography and for spectroscopic investigations on the single crystals
- Crystallization of the ATP-Synthase (F 0F 1) from the thermophilic Cyanobacterium Synechococcus elongatus
Principle investigator: Petra Fromme,funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the priority program 1070 ”Structure and Function of Energy Converting Systems in Procaryonts ” project no: Fr 320/4-1 and Fr420/4-1 Period: 04/15/1999 to 04/14/2003
This grant provided support for structural and functional investigations on the ATP-Synthase, a molecular motor which driven by an electrochemical gradient
- Crystallization of a New Type of Pore-Proteins of the Outer-Chloroplast- Membrane
Principle investigator: Petra Fromme,funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) project no: Fr 320/-3-1 and FR320/3-2 Period: 03/15/1998 to 03/14/2002
This grant provided support for folding studies as well as structural and functional investigations on an amino acid transporter of the outer chloroplast membrane
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