Current funding at Arizona State University
- Establishment of a high throughput protein production center to empower translational research in Arizona
Principle Investigator: Joshua LaBear (co-PI Petra Fromme)
Source of funding: Flinn Foundation 30005389
Funding Period: 10/1/2014-9/30/2018
The major goal of this grant is to establish a high-throughput protein production center at ASU. An in vivo insect cell protein production system will be developed that will facilitate in vivo protein crystallization and structure determination using serial femtosecond nanocrystallography with X-ray free electron lasers.
- Biology with X-ray Free Electron Lasers
Principle Investigator: Eaton Lattman from State University of New York at Buffalo (co-PI Petra Fromme)
Source of funding: NSF 1231306
Funding Period: 8/1/2013-9/30/2018
The major goal of this grant is to utilize the capabilities of X-ray free electron lasers (XFEL), by working to develop the tools and software applications needed to expand this knowledge outward. These applications will be applied to a wide range of scientific inquiry, including the studies of photosynthesis, enzyme function at the atomic level and drug-delivery proteins.
- Femtosecond nano-crystallography of membrane proteins
Principle investigator: P. Fromme
Source of funding: HHS-NIH
Proposed Funding Period: 9/1/2010-8/30/2019
The major goal of this project is to develop the method of femtosecond nanocrystallography, where X-ray data are collected on a stream of fully hydrated nanocrystals using the Free Electron Laser at LCLS
- Center for Membrane Proteins in Infectious Diseases, MPID
Principle investigator: P. Fromme
Source of funding: HHS-NIH-NIGMS
Proposed Funding Period: 10/1/2010-6/30/2016
The major goal of this grant is to determine structures of membrane proteins in infectious diseases and develop new methods for high-throughput structure determination of membrane proteins.
Completed Research Grants
- Structure and function of supercomplexes of Photosystem I with its peripheral antenna systems in green algae and cyanobacteria
Principle investigator: P. Fromme
Source of funding: NSF MCB 04127142
Funding Period: 12/01/2004 to 11/30/2011
The major goal of this grant is the crystallization and structural and functional investigations on the green algae and cyanobacterial PS I in complex with their external antenna systems: the LHC I in green algae and the IsiA antenna in cyanobacteria.
- Structure and function of the proton-turbine of the ATP-synthase
Principle investigator: P. Fromme
Source of funding: NIH R01 GM081490-01
Funding Period: 09/01/2007-08-31-2011
The major goal of this grant is the study of the structure and function of the ATP-Synthase. The ATP-synthase is a molecular motor, which synthesizes ATP driven by a transmembrane proton gradient. The aim of the project is to determine the structure of the intact complete ATP-Synthase thereby un raveling the mechanism of the coupling of proton translocation to ATP synthesis.
- EFR Center for Bio-Inspired Solar Fuel Production
Principle investigator: D. Gust
Source of funding: DOE-U.S. Department of Energy 0903273
Funding Period: 8/1/2009 to 7/31/2014
The major goal of this grant is the study and development of an integrated systems for biosolar energy conversion. Sunlight absorbed by an artificial reaction center will produce electrochemical potential for artificial water oxidation and proton reduction to hydrogen gas at a second peptide-based, electrode-bound catalyst. The part of P.Fromme is focused on the development of an artificial oxygen evolving complex as a biomimetic system for Photosystem II.
- Fluctuation X-ray diffraction of membrane proteins in solution
Principle investigator: P. Fromme
Source of funding: DOE 09083939 subcontract from University of Wisconsin-Milwaukee
Funding Period: 10/1/2009 to 9/30/2012
The major goal of this grant is the development of ultra-fast time resolved Small Angle Scattering for structure determination of membrane proteins in solution
- Dynamics of electron transfer-PSI/ferredoxin
Principle investigator: J. Spence (co-PI Petra Fromme)
Proposed Funding Period: 6/1/2010-5/31/2013
The major goal of this grant is to unravel the conformational changes involved in the inter-protein electron transfer between the membrane protein complex Photosystem I and the soluble electron acceptor protein ferredoxin by light induced time resolved femtosecond crystallography
- The mechanism of water splitting in PS II investigated by X-ray crystallography and spectroscopy
Principle investigator: P. Fromme; co-Pi's R. LeBrutto (ASU, SOLS) and D. Britt (UCLA)
Source of support: NIH R01 GM7 1619-01
Funding period: 07/2004-06/2010
This grant provided support for the investigation of the structure and function of Photosystem II, a large membrane protein complex, which produces all the oxygen in the atmosphere. The protein complex will be crystallized and the structure determination will be performed to atomic resolution by X-ray structure analysis. The structural studies are complemented by functional investigations using Electron Paramagnetic Resonance (EPR) techniques. The aim of the project is to unravel the mechanism of water splitting.
- Interaction of Photosystem I with its natural electron acceptor ferredoxin: a model system for protein recognition and inter-protein electron transfer
Principle investigator: P. Fromme
Source of funding: United States Department of Agriculture 2003-35318-13573
Funding Period 08/2003 to 08/2006
This grant provided support for the study of the interaction of Photosystem I with ferredoxin. The research involves the improvement of the PS I-ferredoxin crystals, X-ray structure analysis as well as EPR spectroscopic investigations on the complex
- NSF98-119 Serial crystallography
Principle investigator: J.C.H. Spence (ASU Physics) coPI's : B. Doak (ASU Physics), : P. Fromme (ASU Chem. and Biochem.), U.Weierstall (ASU Physics), G. Hembree (ASU Physics) , K. Schmidt (ASU Physics)
Source of support: SGER-NSF
This grant provided initial funding for the first steps in the development of a new technique for the structure determination of proteins by electron diffraction of oriented single molecules. The first part of the project includes the development of a stream of nano-heliumparticles, each of them including one protein molecule covered by a shell of amorphous ice. The next step will be the orientation of the particles in a laser beam and the detection of the electron diffraction of the stream of oriented molecules. The diffraction of up to 10 10 oriented molecules will be thereby detected to provide a diffraction pattern. The final aim of the project is to determine of protein- structures by single molecule electron diffraction.
Previous funding at the Technical University Berlin
- Advanced diagnostics and operation concepts for crystal growth of bio-molecules under microgravity
Principle investigators: P. Fromme, N. Chayen and K. Dierks and Partner Systemtechnik, Hamburg funded by the European Space agency SA-Grant
Period: 11/01/2000 to 10/31/2003
This grant provided support for the development of a new type of crystallization-reactor for the crystallization of bio-molecules under microgravity at the International Space Station. The aim of the project is to develop a crystallization reactor that allows full online control of the crystallization parameter in orbit and includes seeding options.
- New Developments for Crystallization of Photosynthetic Membrane Proteins under microgravity
Principle investigator: P. Fromme funded by the Germany Space Agency (Deutsche Gesellschaft fr Luft und Raumfahrt) and the Bundesministerium fuer Forschung BMBF (Minister of Research)
Period: 10/01/2000 to 12/31/2003
This grant provided support for the research on the nucleation and crystallization processes during crystallization of membrane proteins under microgravity. The main aim was to grow large well ordered single crystals of Photosystem I during the STS-107 mission of the space shuttle Columbia.
- Crystallization of the water-oxidizing Photosystem II for structural and functional analysis of the system
Principle investigators: Petra Fromme and H.T Witt, funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the Special Research Unit 498Protein-Cofactor-Interactions in Biological Systems, project C3
Period: 01/01/1999 to 12/31/2002
This grant provided support for structural and functional investigations on the Photosystem II. The project includes crystallization of Photosystem II, X-ray structure analysis and spectroscopic investigations on the single crystals.
- Structure and Function of Photosystem I
Principle investigators: Petra Fromme, Eberhard Schlodder, H.T. Witt funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the Special Research Unit 498Protein-Cofactor-Interactions in Biological Systems, project A1
Period: 01/01/1999 to 12/31/2002
This grant provided support for the structure determination of Photosystem I by the method of X-ray crystallography and for spectroscopic investigations on the single crystals
- Crystallization of the ATP-Synthase (F 0F 1) from the thermophilic Cyanobacterium Synechococcus elongatus
Principle investigator: Petra Fromme, funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) in the priority program 1070 Structure and Function of Energy Converting Systems in Procaryonts project no: Fr 320/4-1 and Fr420/4-1
Period: 04/15/1999 to 04/14/2003
This grant provided support for structural and functional investigations on the ATP-Synthase, a molecular motor which driven by an electrochemical gradient
- Crystallization of a New Type of Pore-Proteins of the Outer-Chloroplast- Membrane
Principle investigator: Petra Fromme, funded by the German Science Foundation (Deutsche Forschungsgemeinschaft) project no: Fr 320/-3-1 and FR320/3-2
Period: 03/15/1998 to 03/14/2002
This grant provided support for folding studies as well as structural and functional investigations on an amino acid transporter of the outer chloroplast membrane
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